WebDownload scientific diagram CircATG4B‐222aa protects against the effect of ATG4B induced by TMED10‐mediated inhibition. A) Total proteins from flag‐circATG4B‐222aa … WebMar 10, 2024 · The physiological interaction between TMED10 and ALK5 prompted us to examine their subcellular colocalization. When human embryonic retinoblast 911 cells were transfected with both TMED10 and ALK5, both of them could be seen mainly in the cytosol (Fig. 1F, upper panels), because both proteins are synthesized in the rough endoplasmic …
TMED10 transmembrane p24 trafficking protein 10
WebSep 9, 2024 · TMED10:GFP is stained in green, GM130 in red and nucleus in blue. Dotted squares are magnified on the right, arrows show TMED10:GFP-only-labeled vesicles. n =3 independent experiments. Scale bars: 10 µm; 5 µm in zoom. (B) Quantification of TMED10 and GM130 colocalization. WebAug 1, 2008 · ... Because phosphorylation of ATG4B stimulates its activity, we elucidated the effect of TMED10 knockdown on ATG4B activity. Using a cell-based assay system wherein the proteolytic activity... baku f1 qualifying times
Down-regulated TMED10 in Alzheimer disease induces autophagy via …
WebFigure Legend Snippet: Downregulation of TMED10 increases the proteolytic activity of ATG4B. ( A ) Schematic diagram of the quantification of ATG4B activity using an assay based on a luciferase-release system. ( B and C ) SH-SY5Y cells transfected with pEAK12-Actin-LC3-dNGLUC were transfected with scrambled siRNA ( Sc ) or TMED10 siRNA ( … WebAlthough the precise mechanism is not fully understood, the authors showed that a LC3B T50E phospho-mimicking mutant also inhibited interactions with ATG4B, ATG7 and syntaxin-17 (STX17). Interestingly, the Thr50 site is conserved among the LC3 family members, but is not present in the GABARAP proteins (Shrestha et al., 2024). WebMar 19, 2024 · Down-regulated TMED10 in Alzheimer disease induces autophagy via ATG4B activation. ... are hair bandanas 90s