Webb24 juli 2015 · We show that thermostabilizing mutations in T7 RNA polymerase are able to act orthogonally and in an additive way to the substrate specificity mutations, rescuing … Webb22 dec. 2015 · To identify a human SERT (hSERT) construct suitable for detailed biochemical and structural studies, we developed an efficient thermostability screening protocol and rapidly screened 219 mutations for thermostabilization of hSERT in complex with the SSRI paroxetine.
(PDF) The effect of thermostabilising mutations on the pressure ...
WebbStreptomyces mobaraenesis transglutaminase can catalyze the cross-linking of proteins, which has been widely used in food processing. In this study, we rationally modified flexible regions to further improve the thermostability of FRAPD-TGm2 (S2P-S23V-Y24N-E28T-S199A-A265P-A287P-K294L), a stable mutant of the transglutaminase constructed in … Webb15 juni 2012 · Phrased differently, WSMR r > 1 means that CNA successfully identified those (structurally weak) parts of CS where (thermostabilizing) mutations occur more … food hygiene posters printable
Structure Determination of Inactive-State GPCRs with a Universal ...
Webb1 mars 2011 · The mutations included the replacement of an irregular loop by a shorter variant and the introduction of a ten–residue (3– hairpin. In general, these drastic mutations had little effect on the production and activity of NP–sub, indicating the feasibility of major structural rearrangements at the surface of proteins. Webb11 apr. 2024 · The decrease of antibody efficacy to mutated SARS-CoV-2 spike RBD explains the breakthrough infections and reinfections by Omicron variants. Here, we analyzed broadly neutralizing antibodies ... Webb23 dec. 2009 · The mutations resulted in a minor decrease in the Gibbs free energy change of the TcSOD dissociation, while a significant decrease in that of the unfolding of the monomeirc intermediate. These results herein suggested that the additional ion-pairing network at the C- terminus mainly enhanced the structural stability of hyperthermophilic … elden ring was mohg cheese patched